| Zika MAC ELISA Verification Panel |
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| Laboratory Name: |
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| Primary Contact: |
Name: |
Phone: |
Email: |
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| Address: |
City: |
State: |
Zip: |
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| Testing Personnel Initials |
Initial Here: |
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| Test Date: |
Date Here: |
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| Test Method. Manual, Dynex Agility, or DSX |
Enter Test Method Here: |
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| Reagents and Consumables |
Source |
Catalog # |
Lot # |
Working Dilution |
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| Goat anti-human IgM |
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| Zika Vero E6 Tissue Culture Antigen |
CDC |
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| Normal Vero E6 Antigen |
CDC |
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| Zika COS-1 Recombinant Antigen |
CDC |
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| Normal Control COS-1 Recombinant Antigen |
CDC |
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| Detecting antibody conjugate: Horseradish peroxidase conjugated monoclonal antibody 6B6C-1 |
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| Immulon II HB flat-bottomed 96 well plates |
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| Enhanced K-Blue TMB substrate |
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| Flavivirus IgM Positive Contol |
CDC |
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| Calibration Control Serum |
CDC |
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| Negative Control Serum |
CDC |
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| Panel Results |
| Sample |
Viral Antigen OD |
Mean Viral Antigen O.D. |
Normal Antigen OD |
Mean Normal Antigen O.D. |
P/N Values |
Assay Interpretation |
| OD1 |
OD2 |
OD3 |
OD1 |
OD2 |
OD3 |
P/N Within Sample* |
P/N of Test Sample** |
| S1 |
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| S2 |
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| S3 |
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| S4 |
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| S5 |
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| S6 |
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| S7 |
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| Flavivirus IgM Positive Contol |
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| Calibration Control Serum |
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| Negative Control Serum |
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| * P/N Within Sample = mean OD of test sample reacted with Zika Antigen divided by the mean OD of test sample reacted with the Normal Antigen |
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| ** P/N of Test Sample = mean OD of test sample reacted with the Zika antigen divided by the mean OD of the Calibration Control Serum reacted with the Zika Antigen |
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