Form 1 DS Rodent Survey

Research Survey: Down Syndrome Rodent Models

The purpose of this survey is to learn how scientists use rodent models for DS research; assess satisfaction with currently
available models; describe researchers' perceptions of the limitations of currently available models; and provide information
to help assess future needs for such research resources.
The survey should take less than 10 minutes to complete, on average. Thank you for participating in our survey. Your
feedback is important.
-------OMB#: 0925-0643
ExpDate:10/2017

Public reporting burden for this collection of information is estimated to average 10 minutes per response, including the time
for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and
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estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to: NIH, Project
Clearance Branch, 6705 Rockledge Drive, MSC 7974, Bethesda, MD 20892-7974, ATTN: PRA (0925-0643). Do not return
the completed form to this address.

Research Survey: Down Syndrome Rodent Models

1. Are you currently conducting research on Down syndrome using
animal (rodent) models?
Yes
No
Not sure

2. If you use rodent models for Down syndrome, which one(s) do you use? (check
apply)
Ts65Dn (sighted). (B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ): Cesium irradiation was used to produce a reciprocal translocation, T (16;17) 65Dn. This strain does
retinal degeneration gene.

Ts65Dn (can lose vision). (B6EiC3Sn a/A-Ts(1716)65Dn/J): Cesium irradiation was used to produce a reciprocal translocation, T (16;17) 65Dn; this strain con
degeneration gene.

Ts1Cje. (B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDnJ): This strain carries a Robertsonian fusion between the small Ts65Dn marker chromosome and Chr 12.
of Chr 16 genes from App to Mx1, the same as in Ts65Dn strains. This strain contains the retinal degeneration gene.

Dp(16)1Yey. (B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J): This strain was engineered to contain a duplication orthologous to human 21q11-q22.3 and carries 113 ge
to genes on Hsa21. This strain does not contain the retinal degeneration gene.

Dp(10)1Yey. (B6;129-Dp(10Prmt2-Pdxk)2Yey/J): This strain was engineered to contain a duplication of 41 genes syntenic to the distal part of human 21q22.3

Dp(17)1Yey. (B6;129-Dp(17Abcg1-Rrp1b)3Yey/J): This strain was engineered to contain a duplication of 19 genes syntenic to the proximal part of human 21q
Tc1. (B6129S-Tc(HSA21)1TybEmcf/J): This strain carries a freely segregating chromosome containing 90% of HSA21 (269 genes).
Other (please specify)

3. How well do these mouse models above satisfy your current research
needs?
Not at all

Not very well

Somewhat well

Very well

Extremely well

4. What suggestions would you make to improve upon the existing
strains?

5. Are you doing any research involving rodent models of a single gene
alteration on human chromosome 21 (e.g., transgenic, targeted deletion,
duplication of App, Dyrk1a, etc.)?
Yes
No

Research Survey: Down Syndrome Rodent Models

6. Which gene(s) are you altering on chromosome 21?

Research Survey: Down Syndrome Rodent Models

7. Please describe any existing rodent Down syndrome models that you
would like to see be made available to the investigator community. If
none, please enter "none".

8. Please describe any new models that you think should be engineered
and made available to investigators. If none, please enter "none".

9. Please describe any rodent models of Down syndrome that you have
generated that you would like to make available to other investigators?
(If none, please enter "none").

10. Please describe any specific BAC transgenic lines you think should
be made available? (If none, please enter "none").

11. Do you consider genetic background when you choose mouse
models and/or design your experiments?
Yes
No

Research Survey: Down Syndrome Rodent Models

12. Please describe how you consider genetic background when you
choose mouse models and/or design your experiments.

Research Survey: Down Syndrome Rodent Models

13. Where do you receive funding for your Down syndrome rodent
research? (check all that apply)
NIH
European Commission funding
The Wellcome Trust
Private Foundation (fill in name):________________________________________________________
Other (fill in name):___________________________________________________________________

14. From which resource do you receive your rodent models? (check all that appl
Jackson Laboratory (JAX)/Charles River Laboratory
Taconic Biosciences
European Mouse Mutant Archive (EMMA)
Directly from collaborators
Other (please specify)

15. Additional comments or suggestions:

16. If we have further questions, can we contact you for more details?
(optional)
Name
Email