Biospecimen Breast Millk Collection Instruments (1, 3 MO)

OMB #: 0925-0593

OMB Expiration Date: 07/31/ 2013

Biospecimen Breast Milk Collection Instructions

The National Children’s Study

Breast Milk Collection Instructions

As part of the National Children’s Study, we are asking you to collect a sample of your breast milk. We are asking you to provide 80 mL (about 3 ounces) of breast milk.

The supplies included in the Breast Milk Collection Kit are:

• 2 absorbent cloths

• 1 plastic collection container, prelabeled

• 1 plastic amber bag with absorbent pad

A Breast Milk Collection Self-Administered Questionnaire (SAQ) will be provided to record information about the collection.

Important Instructions Before Getting Started

• Please review all information before beginning the collection. For this sample collection, you are asked to collect breast milk from one breast only and to completely empty the breast. If your baby is hungry and needs to be fed soon after you have collected the sample, use the other breast.

• Please do not breast-feed your baby or pump breast milk from the breast you will use to provide this sample for 2 hours prior to the sample collection.
  
  

• Please do not eat food or take medication for 2 hours prior to the sample collection.
  
  

• Please collect the breast milk sample sometime in the morning, preferably between 9:00 a.m. – 12:00 p.m. (noon). You will be asked to write down the time you collected the sample on the Breast Milk Collection SAQ.
  
  

• Please use your own breast pump to collect this sample. If you choose, you may also squeeze out the breast milk by hand in order to collect this sample.
  
  

• Please do not use any soaps, cleansers, lotions, or creams on the breast or nipple for at least 2 hours prior to the sample collection.
  
  

• Be sure to drink plenty of water before and after the sample collection.

Collection Instructions

1. Thoroughly wash your hands with soap and water and dry with a clean towel.

2. Wash one breast and nipple with warm tap water only using one of the two absorbent cloths provided. Please do not use any soap or other cleanser to clean the area, and do not apply any lotion or cream. Gently dry the area with the second absorbent cloth.

3. Follow the manufacturer’s instructions for your breast pump in order to safely collect the sample.

4. If you choose to squeeze out the breast milk by hand, you can collect the milk directly in the plastic container provided.

Immediately After Collection

1. Gently mix the milk in the container you used to collect the sample by swirling the container. This is important in order to thoroughly mix the different components of the breast milk.

2. Remove the lid from the prelabeled plastic container provided in the Breast Milk Collection Kit. After you have gently mixed the sample, pour the milk into the container so that the total volume in the container is approximately to the 80 mL mark on the container. If you did not collect enough milk to fill the container to the 80 mL mark, pour whatever you have collected into the container. Place the lid on the container.

3. If there is any milk left over in the container you used to collect the sample, you can save it for your baby.

4. Place the prelabeled plastic container into the plastic amber bag with the absorbent pad, seal the bag, and place it in the freezer until it can be picked up by the Study Center staff.

5. Complete the Breast Milk Data Collection SAQ as soon as possible after collection.

6. Contact Study Center staff to arrange for pickup of the sample.

Please call the Study Center number on the last page, if you have any questions.

Thank you for providing this sample!

For Office Use Only:

Insert local contact information label here.

Insert participant ID label here.

Public reporting burden for this collection of information is estimated to average 5 minutes per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. An agency may not conduct or sponsor, and a person is not required to respond to, a collection of information unless it displays a currently valid OMB control number. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to: NIH, Project Clearance Branch, 6705 Rockledge Drive, MSC 7974, Bethesda, MD 20892-7974, ATTN: PRA (0925-0593*). Do not return the completed form to this address.

BIO Breast Milk Collection Instructions (EHPBHI), MDES 3.0 Spring 2012, V1.0 1