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ATTACHMENT 20

PLANS FOR TABULATION AND ANALYSIS AND PUBLICATION
OF THE NESARC-III DATA

Table 1. Tabulation Plans for the NESARC-III Data

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1. Prevalence of major outcome variables cross-classified by:
(1) one another; and (2) demographic, socioeconomics, and environmental variables

Major Outcome Variables

A. Alcohol consumption levels (e.g., quantity, frequency, duration, and volume) and drinking patterns (e.g., binge drinking and episodic drinking)

B. Alcohol use disorders and their associated disabilities

2. Demographic and Socioeconomic Variables (Cross-Tabulation Variables)

A. Age

B. Race-ethnicity

C. Sex

D. Marital status

E. Personal income

F. Household income

G. Education

H. Geographic region of residence

I. Population density of residence

J. Employment status

K. Occupation

L. Adverse childhood events

M. Stressfull life events

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Table 2. Weighted Allele Frequencies of Genetic Variants in the US Population by Race-Ethnicity

Gene Symbol	Gene Name [Chromosome Position]	Pathway	Variant	Nucleotide Position [Amino Acid Change]	Allele	Total US, %	Non-Hispanic White, %	Non-Hispanic Black, %	Mexican American	p value
ABCB1	ATP-binding cassette, subfamily B (MDR/TAP), member 1[7q21.1]	13	rs1045642		T (C)
ACE	Angiotensin 1 converting enzyme (peptidyl-dipeptidase A) 1[17q23.3]	2, 12	rs4646994		ins(del)
ADH1B	Alcohol dehydrogenase IB (class I), beta polypeptide [4q21-q23]	12, 13	rs1229984 rs17033 rs2066702		A(G) G(A) T(C)
ADH1C	Alcohol dehydrogenase IC (class I), gamma polypeptide [4q21-q23]	12, 13	rs1693482 rs698		A(G) G(A)
ADRB1	Adrenergic, beta-1- receptor [10q24-q26]	2, 3, 12	rs1801252		G(A)
ADRB2	Adrenergic, beta-2- receptor, surface [5q311-q32]	2, 3, 12	rs1042713 rs1042714		A(G) G(C)
ADRB3	Adrenergic, beta-3- receptor [8p12-p11.2]	12	rs4994		C(T)
ALAD	Aminolevulinate, delta- dehydratase [9q33.1]	12, 13	rs1800435		C(G)

Table 3. Results of Logistic Regression Analyses of Alcohol Dependence^a

	E uropean American		A frican American		Pooled
	OR (95% CI)	P Value	OR (95% CI)	P Value	OR (95% CI)	P Value
Sex
Age, y
Ancestry proportion score
Adult traumatic events
Childhood adversity
DRD2 genotype
DRD2 × adult traumatic events
DRD2 × childhood adversity

Abbreviations: OR odds ratio; CI, confidence interval.

^a Sex, age, adult traumatic events, and childhood adversity will be used as covariates in all the models. In addition, ancestry proportion scores will be included in the pooled model.

Table 4. Allele and Genotype Frequencies of CNR1 Markers in European Americans

Markers	Allele or Genotype	C ontrol		D D&AD		D D		A D		Total SD
		n	f	n	f	n	f	n	f	n	f
SNP1	C
rs1884830	G
	CC
	CG
	GG
SNP2	A
rs2180619	G
	AA
	AG
	GG
SNP3	T
rs6454674	G
	TT
	TG
	GG
SNP4	A
rs806379	T
	AA
	AT
	TT
SNP5	T
rs806377	C
	TT
	TC
	CC
SNP6	T
rs806371	G
	TT
	TG
	GG
SNP7	A
Rs1049353	G
	AA
	AG
	GG
SNP8	T
rs806368	C
	TT
	TC
	CC
SNP9	T
rs806365	C
	TT
	TC
	CC
SNP10	T
rs2146274	C
	TT
	TC
	CC

DD&AD, comorbid DD and AD; DD, drug dependence; AD, alcohol dependence; Total-SD, all the cases in the present study; n, number of chromosomes (for alleles) or individuals (for genotypes); f, frequency.

^ap≤.05, ^bp≤.05, ^cp≤.007, (= α) for conventional case-control comparison.

^dp≤.05, ^ep≤.05, ^fp≤.007, (= α) for structured association (SA) analysis using STRAT.

Table 5. Regression Analysis on the Association between SNP3 × SNP8 Interaction and Phenotypes in European Americans

G enotype Model

Mixed Model

95% CI for OR

95% CI for OR

Phenotype

Covariates

β

p

OR

Lower

Upper

Covariates

β

p

OR

Lower

Upper

DD&AD

Constant

Constant

Sex

Sex

Age

Age

SNP3 × SNP8

SNP3 × SNP8

SNP3^G/G × SNP8^T/T

SNP3^G/T × SNP8^T/T

SNP3^G/T × SNP8^T/C

SNP3^G/G × SNP8^T/C

DD

Constant

Constant

Sex

Sex

Age

Age

SNP3 × SNP8

SNP3 × SNP8

SNP3^G/G × SNP8^T/T

SNP3^G/T × SNP8^T/T

SNP3^G/T × SNP8^T/C

SNP3^G/G × SNP8^T/C

AD

Constant

Constant

Sex

Sex

Age

Age

SNP3

SNP3 × SNP8

SNP3^G/G

SNP3^G/T

SNP3 × SNP8

Total SD

Constant

Constant

Sex

Sex

Age

Age

SNP3 × SNP8

SNP3 × SNP8

SNP3^G/G × SNP8^T/T

SNP3^G/T × SNP8^T/T

SNP3^G/T × SNP8^T/C

SNP3^G/G × SNP8^T/C

DD&AD, DD, AD and Total. Phenotypes, Covariates, Genotype model, β, OR, CI, and E – n. Mixed model, the genotypes of SNP3 and SNP8 are in an additive and recessive
models respectively in the regression model, that is, SNP3^T/T = 0, G/T = 1, G/G = 2, SNP8^C/C = C/T = 0, and SNP8^T/T = 1.